You might take a look at the link below. Seems to be helping a lot of other people.
Vista Limewire Fix Once And For All!!!
Try these fixes if above is no help.
Try uninstalling all versions of Java from the add/remove programs and then re-install the JRE 5.11 offline version....
Archive: Download Java 2 Platform Standard Edition (J2SE) 5.0 Update 11
If that does not help with your problem try deleting your preference folder/s...
Fixes for Limewire when LW isn't operating as it should LimeWire Pref Folder in Vista
Supply these details If all else fails.
System details - help us to help you -> Provide these details when you post!!!.